Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures
Identifieur interne : 003270 ( Main/Exploration ); précédent : 003269; suivant : 003271Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures
Auteurs : R. Ghorbel [Espagne] ; L. Navarro [Espagne] ; N. Duran-Vila [Espagne]Source :
- Plant pathology [ 0032-0862 ] ; 1998.
Descripteurs français
- Pascal (Inist)
- Culture tissu, Différenciation, Indexage plante, Morphogenèse, Propriété biologique, Régénération, Symptomatologie, Isolat, Souche, Plante hôte, Citrus aurantifolia, Citrus macrophylla, Citrus paradisi, Citrus sinensis, Virus tristeza agrume, Etude expérimentale, Etude méthode, Espagne, Caractérisation biologique.
- Wicri :
- geographic : Espagne.
English descriptors
- KwdEn :
- Biological properties, Citrus aurantifolia, Citrus macrophylla, Citrus paradisi, Citrus sinensis, Citrus tristeza virus, Differentiation, Experimental study, Host plant, Isolate, Method study, Morphogenesis, Plant indexing, Regeneration, Spain, Strain, Symptomatology, Tissue culture, biological characterization.
Abstract
Stem segments from Mexican lime, sweet orange, grapefruit, Citrus excelsa and alemow, infected with five citrus tristeza virus (CTV) isolates, were cultured in vitro. Regeneration of roots and shoots were modified as a result of infection. The effect of CTV on the morphogenesis of stem segments cultured in vitro depended on the CTV isolate and the plant host, and showed a correlation with the in vivo effects observed in biological indexing. Evaluation of the morphogenic response of stem segments of Mexican lime and grapefruit can be used as an additional tool for the biological characterization of CTV isolates. The symptoms on sweet orange plants obtained from regenerated shoots indicated that CTV is unevenly distributed in the host plant cells and that the regeneration process may be utilized as a tool to separate strains from complex field isolates.
Affiliations:
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Le document en format XML
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Navarro</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado oficial</s1><s2>46113 Moncada, Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46113 Moncada, Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado oficial</s1><s2>46113 Moncada, Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46113 Moncada, Valencia</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">98-0386004</idno><date when="1998">1998</date><idno type="stanalyst">PASCAL 98-0386004 INIST</idno><idno type="RBID">Pascal:98-0386004</idno><idno type="wicri:Area/PascalFrancis/Corpus">000A78</idno><idno type="wicri:Area/PascalFrancis/Curation">001429</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000A83</idno><idno type="wicri:doubleKey">0032-0862:1998:Ghorbel R:biological:characterization:of</idno><idno type="wicri:Area/Main/Merge">003515</idno><idno type="wicri:Area/Main/Curation">003270</idno><idno type="wicri:Area/Main/Exploration">003270</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Biological characterization of citrus tristeza virus isolates by in vitro tissue cultures</title><author><name sortKey="Ghorbel, R" sort="Ghorbel, R" uniqKey="Ghorbel R" first="R." last="Ghorbel">R. Ghorbel</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado oficial</s1><s2>46113 Moncada, Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46113 Moncada, Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado oficial</s1><s2>46113 Moncada, Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46113 Moncada, Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado oficial</s1><s2>46113 Moncada, Valencia</s2><s3>ESP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ></inist:fA14><country>Espagne</country><wicri:noRegion>46113 Moncada, Valencia</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Plant pathology</title><title level="j" type="abbreviated">Plant pathol.</title><idno type="ISSN">0032-0862</idno><imprint><date when="1998">1998</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant pathology</title><title level="j" type="abbreviated">Plant pathol.</title><idno type="ISSN">0032-0862</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological properties</term><term>Citrus aurantifolia</term><term>Citrus macrophylla</term><term>Citrus paradisi</term><term>Citrus sinensis</term><term>Citrus tristeza virus</term><term>Differentiation</term><term>Experimental study</term><term>Host plant</term><term>Isolate</term><term>Method study</term><term>Morphogenesis</term><term>Plant indexing</term><term>Regeneration</term><term>Spain</term><term>Strain</term><term>Symptomatology</term><term>Tissue culture</term><term>biological characterization</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Culture tissu</term><term>Différenciation</term><term>Indexage plante</term><term>Morphogenèse</term><term>Propriété biologique</term><term>Régénération</term><term>Symptomatologie</term><term>Isolat</term><term>Souche</term><term>Plante hôte</term><term>Citrus aurantifolia</term><term>Citrus macrophylla</term><term>Citrus paradisi</term><term>Citrus sinensis</term><term>Virus tristeza agrume</term><term>Etude expérimentale</term><term>Etude méthode</term><term>Espagne</term><term>Caractérisation biologique</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Espagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Stem segments from Mexican lime, sweet orange, grapefruit, Citrus excelsa and alemow, infected with five citrus tristeza virus (CTV) isolates, were cultured in vitro. Regeneration of roots and shoots were modified as a result of infection. The effect of CTV on the morphogenesis of stem segments cultured in vitro depended on the CTV isolate and the plant host, and showed a correlation with the in vivo effects observed in biological indexing. Evaluation of the morphogenic response of stem segments of Mexican lime and grapefruit can be used as an additional tool for the biological characterization of CTV isolates. The symptoms on sweet orange plants obtained from regenerated shoots indicated that CTV is unevenly distributed in the host plant cells and that the regeneration process may be utilized as a tool to separate strains from complex field isolates.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><country name="Espagne"><noRegion><name sortKey="Ghorbel, R" sort="Ghorbel, R" uniqKey="Ghorbel R" first="R." last="Ghorbel">R. Ghorbel</name></noRegion><name sortKey="Duran Vila, N" sort="Duran Vila, N" uniqKey="Duran Vila N" first="N." last="Duran-Vila">N. Duran-Vila</name><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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